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EDTA Blood Collection Tube with Precise Anticoagulation

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EDTA Blood Collection Tube with Precise Anticoagulation

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Brand Name : Orsin

Model Number : Gel Clot ActivatoR

Certification : ISO 13485

Place of Origin : China

MOQ : 100/pcs

Price : Negotiation

Payment Terms : L/C,D/A,D/P,T/T,Western Union

Supply Ability : 20million/pcs/moth

Delivery Time : 1-4 weeks

Shipping Options : Sea, air, or train

Lead Time : 1-4 weeks

Logo Printing : Available for big orders (>30000 PCS)

Packaging Material : PET/Glass

Color : Transparent

Advantages : Enhanced Stability

Anticoagulant Properties : Excellent

Storage Condition : Store at room temperature

Clinical Use : Blood bank

Properties : Durable and reliable

Clolor : purple

Sample Order : Available

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I. Product Characteristics

EDTA blood collection tubes (purple cap) utilize dipotassium or tripotassium ethylenediaminetetraacetate (EDTA-K₂ or EDTA-K₃) as the core anticoagulant, featuring:

  1. Precise Anticoagulation Mechanism: Chelates calcium ions (Ca²⁺) in blood to block thrombin activation, achieving complete anticoagulation while maximally preserving blood cell morphology.

  2. Optimized Physical Design:

    • High-transparency PET plastic or glass tube with mechanical strength and light resistance;

    • Pre-filled precise anticoagulant dosage (1.5–2.2 mg EDTA/mL blood), vacuum accuracy ≤±5%.

  3. Safety & Quality Control:

    • Gamma-irradiated sterilization (8–25 kGy), controlled endotoxin levels;

    • ISO13485/CE compliant; standardized purple cap for rapid identification.

II. Application Scenarios

Primarily for hematological testing, with specific applications:

Test Category Suitable Applications Restrictions
Basic Hematology Complete Blood Count (CBC), Hemoglobin (Hb), Blood Typing, Reticulocyte Count Coagulation Tests (e.g., PT/APTT), Platelet Function Testing
Specialized Tests Glycated Hemoglobin (HbA1c), Hemoglobin Electrophoresis, Flow Cytometry Electrolytes (Ca²⁺/K⁺/Na⁺)/Iron Assays, Alkaline Phosphatase, Creatine Kinase
Molecular Biology DNA/RNA Extraction, Pathogen Nucleic Acid Testing (with stabilizers) PCR (EDTA may inhibit enzyme activity)

Note: EDTA tubes cannot be used for electrolyte, trace element, or coagulation testing due to chelation interference.

III. Usage Protocol & Specifications

Procedure Requirements Key Operational Notes
Pre-collection Check expiration date/vacuum integrity Storage: 2–25°C, dark, dry conditions
Collection & Mixing Gently invert 5–8 times immediately post-draw Excessive force → hemolysis; insufficient mixing → microclots; accelerate if >25°C
Testing Window Routine tests: complete within 24h WBC count: 48h at 4°C; Platelet count: ≤24h at 4°C
Centrifugation Not for direct centrifugation Plasma separation requires gel separator (0.8–1.2 g/tube)

IV. Precautions

  1. Anticoagulant Risks:

    • Overfill (>2.2 mg/mL): RBC shrinkage/platelet swelling → morphology distortion;

    • Underfill: Inadequate anticoagulation → microclots → instrument obstruction.

  2. Sample Handling:

    • Do not reuse tubes or mix additives;

    • Blood volume ≤ labeled capacity (e.g., ≤5.5 mL for 5 mL tube).

  3. Safety:

    • EDTA is corrosive; rinse skin/eyes immediately with water if contacted.

V. FAQs

  1. EDTA-K₂ vs. EDTA-K₃?

    • Solubility: K₃ dissolves easier → liquid pre-filled tubes; K₂ → spray-dried plastic tubes;

    • Performance: Comparable anticoagulation, but K₃ excess may cause platelet satellitism (pseudothrombocytopenia).

  2. Causes of Pseudothrombocytopenia? Solutions?

    • Causes: EDTA-induced platelet aggregation (~0.1% incidence) or neutrophil adhesion (satellitism);

    • Resolution: Recollect using sodium citrate tube.

  3. Can tubes with microclots be used?
    → No. Microclots indicate anticoagulation failure → recollect with thorough mixing.

  4. Suitable for glucose testing?
    → Compatible with sodium fluoride (2–4 mg/mL blood) to inhibit glycolysis; validate system compatibility.

Clinical Note: EDTA tubes remain the gold standard for CBC. For abnormal cases (e.g., suspected platelet disorders), parallel testing with alternative anticoagulants is recommended.


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